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Before NMR analysis, the sample was reconstituted in 50 l of precooled 4 C methanol, centrifuged at 18,500 g for 10 minutes at 4 C and the resulting supernatant was transferred into a precooled 2 ml Eppendorf and then stored at -80 C [25]

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On the other hand, addition of 30 mM GSSG, an oxidized form of GSH which has no antioxidant properties but is intrinsically acidic, resulted in a reduction in biofilm viability comparable to or greater than 30 mM GSH at intrinsic pH levels (Figure 5B)

doi: 10.1007/978-94-024-1079-2_11

Indeed, Jurat et al., 2007 Conducted a study using 20% honey solution eye drops on human patients with dry eye syndrome